Displacement of tracer by ADP disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
The instrument validation program is a collaborative effort with the major suppliers of multimode readers to ensure that Transcreener® assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance. We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.
| Supplier | Instrument | TR-FRET Assays ADP2, AMP2/GMP2, GDP, UDP, AptaFluor SAH |
||
|---|---|---|---|---|
 |
TriStar²S LB 942 | validated | ||
| Mithras² LB 943 | validated | |||
 |
Cytation™ 5 | validated | ||
| Cytation™ 3 | validated | |||
| Cytation™ 1 | validated | |||
| Synergy™ H1 | validated | |||
| Synergy™ 2/H4/4 | validated | |||
| Synergy™ HTX | not capable | |||
| Synergy™ Neo 2 | validated | |||
 |
POLARstar® Omega | validated | ||
| FLUOstar® Omega | validated | |||
| PHERAstar® FSX | validated | |||
| PHERAstar® Plus/FS | validated | |||
| CLARIOstar® /Plus | validated | |||
| VANTAstar™ | validated | |||
 |
Analyst® GT/HT | validated | ||
| Gemini® XPS/EM | not capable | |||
| SpectraMax® M2/M2e | not capable | |||
| SpectraMax® M5/M5e/FlexStation® 3 | validated | |||
| SpectraMax® Paradigm | contact us | |||
 |
EnVision®/EnVision® Xcite | validated | ||
 |
Infinite® M1000/M1000Pro/Safire2™ | validated | ||
| Infinite® M200 | not capable | |||
| Infinite® F500 | validated | |||
| Infinite® F200/Ultraevolution | contact us | |||
| Spark™ 10M | validated | |||
Validation Criteria
Look for these stickers on instruments that have successfully met the validation criteria.
