Transcreener® ADP2 TR-FRET Assay

The Simplest, Most Direct ADP Detection Method Available … Use for any kinase or ATPase.

Universal ADP Detection with a Far-Red TR-FRET Readout …

The Transcreener® ADP² TR-FRET Assay produces a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) signal that is less sensitive to interference from fluorescent compounds than other detection modes. It is a simple, one-step homogeneous detection assay that accommodates a broad range of ATP concentrations (0.1 to 1,000 µM ATP) and produces excellent signal (Z´ > 0.7 at 10% conversion of 1 µM ATP). The assay can be used for virtually any ADP-producing enzyme including kinases and ATPases.

Displacement of tracer by ADP disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.

UNIVERSAL: detect any ADP-producing enzyme, using any substrate.
SINGLE ADDITION: one-step, simple mix-and-read format.
SENSITIVE: Z’ > 0.7 at low ATP conversion.
LOW INTERFERENCE: far red, time-resolved signal.

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Cat. No.	Product Description	Price	Add to Cart
3011-1K	Transcreener ADP² TR-FRET Red Assay (1,000 Assay, 384 Well)	$699.00	Add to Cart
3011-10K	Transcreener ADP² TR-FRET Red Assay (10,000 Assay, 384 Well)	$3,989.00	Add to Cart
3011-100K	Transcreener ADP² TR-FRET Red Assay (10 X 10,000 Assay, 384 Well)	$19,945.00	Add to Cart

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to ensure that Transcreener^® assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance. We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

Supplier	Instrument	TR-FRET Assays ADP², AMP²/GMP², GDP, UDP, AptaFluor SAH
	TriStar²S LB 942	validated
	Mithras² LB 943	validated

	Cytation™ 5	validated
	Cytation™ 3	validated
	Cytation™ 1	validated
	Synergy™ H1	validated
	Synergy™ 2/H4/4	validated
	Synergy™ HTX	not capable
	Synergy™ Neo 2	validated

	POLARstar^® Omega	validated
	FLUOstar^® Omega	validated
	PHERAstar^® FSX	validated
	PHERAstar^® Plus/FS	validated
	CLARIOstar^® /Plus	validated

	Analyst^® GT/HT	validated
	Gemini^® XPS/EM	not capable
	SpectraMax^® M2/M2e	not capable
	SpectraMax^® M5/M5e/FlexStation^® 3	validated
	SpectraMax^® Paradigm	contact us

	EnVision^®/EnVision^® Xcite	validated

	Infinite^® M1000/M1000Pro/Safire2™	validated
	Infinite^® M200	not capable
	Infinite^® F500	validated
	Infinite^® F200/Ultra^evolution	contact us
	Spark™ 10M	validated