Displacement of tracer by ADP disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
- UNIVERSAL: detect any ADP-producing enzyme, using any substrate.
- SINGLE ADDITION: one-step, simple mix-and-read format.
- SENSITIVE: Z’ > 0.7 at low ATP conversion.
- LOW INTERFERENCE: far red, time-resolved signal.