Transcreener® ADP2 Assay Kit – TR-FRET Readout

Learn Why Direct ADP Detection is The Best HTS Assay Method for Kinases & ATPases

Transcreener ADP2 Assay - TR-FRET Readout

The Transcreener® ADP2 Assay with TR-FRET readout uses displacement of a tracer by ADP to measure enzymatic activity. This disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering. 

The assay produces an excellent signal at <10% ATP consumption for a broad range of initial ATP concentrations (0.1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs. The universal detection method allows screening of virtually any kinase in a high throughput format. 

Used in over millions of well by pharma since 2006, Transcreener is the most extensively validated ADP detection method available backed by the best technical support in the industry. 

Direct Detection of ADP to Measure Kinase Enzymatic Activity

Time-Resolved Förster Resonance Energy Transfer (TR-FRET)

ADP Assay Schematic - TR-FRET Readout

Applications

  • Measure enzymatic activity of kinases
  • Screen compound libraries for kinase modulators
  • Quantify inhibitor potency
  • Inhibitor selectivity profiling
  • Measure drug-target residence time

Features

  • Direct detection of unlabeled ADP
  • Robust Assay Z' > 0.7 under initial velocity conditions
  • Far-red fluorescent readouts minimize compound interference
  • A safe, non-radioactive method
  • Use with ATP concentrations from 0.1 to 1000 µM
  • Universal - use with virtually any kinase

Easy-to-Use, Mix-&-Read, HTS Ready

Run your enzyme reaction, add Transcreener reagents, and read your plates. The ADP assay is compatible with 96, 384, and 1536-well formats. 

Biochemical ATPase Assay Steps

Example Applications

Enzyme
ADKRIPK2
AMPKTBK1
IKK-beta
IRAK4
JAK1
JAK3
MAPK8
PKR
RIPK1

Detection of ADP to Measure MAPK8 Enzymatic Activity with TR-FRET Readout

Below is an example of Transcreener's ADP2 Assay with TR-FRET readout detecting ADP released by MAPK8.

MAPK8 Assay TR-FRET Readout Data

Far Red TR-FRET Readouts Validated on Major Multimode Readers

SupplierInstrumentTR-FRET Assays
TriStar2S LB 942Click Me ArrowValidated
Mithras2 LB 943Click Me ArrowValidated
CytationTM 5Validated
CytationTM 3Validated
CytationTM 1Validated
SynergyTM H1Validated
SynergyTM 2/H4/4Click Me ArrowValidated
SynergyTM HTXNot Capable
SynergyTM Neo 2Validated
POLARstar® OmegaClick Me ArrowValidated
FLUOstar® OmegaClick Me ArrowValidated
PHERAstar® FSXClick Me ArrowValidated
PHERAstar® Plus/FSClick Me ArrowValidated
CLARIOstar® /PlusClick Me ArrowValidated
VANTAstarTMClick Me ArrowValidated
SenseIn Review
Analyst® GT/HTClick Me ArrowValidated
Gemini® XPS/EMNot Capable
SpectraMax® M2/M2eNot Capable
SpectraMax® M5/M5e/FlexStation® 3Click Me ArrowValidated
SpectraMax® ParadigmClick Me ArrowContact Us
SpectraMax® iD3/iD5Click Me ArrowValidated
EnVision®/EnVision® XciteClick Me ArrowValidated
Infinite® M1000/M1000Pro/Safire2TMClick Me ArrowValidated
Infinite® M200Not Capable
Infinite® F500Click Me ArrowValidated
Infinite® F200/UltraevolutionClick Me ArrowContact Us
SparkTM 10MClick Me ArrowValidated

Validation Criteria for Plate Readers

  • 384-Well Format
  • Z'-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
  • Z' specifications to be met using Transcreener ADP2 Assay Reagents
  • Read time to achieve Z' specifications ≤ 5 minutes

Look for these stickers on instruments that have successfully met the validation criteria. 

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine optimal filters and settings for maximal assay performance. We provide you with application notes with all the important details, so you can be confident of success using Transcreener assays on the instrument of your choice. 

Transcreener Instrument Validation

What's Included

What You Will Need

Transcreener ADP2 Assay Kits - TR-FRET Readout

*For custom or bulk orders (over 100,000 wells), please contact us (info@bellbrooklabs.com) for a quote.

Cat. No.Product DescriptionPriceAdd to Cart
3011-1KTranscreener ADP2 TR-FRET Red Assay (1,000 Assay, 384 Well)$789.00Add to Cart
3011-10KTranscreener ADP2 TR-FRET Red Assay (10,000 Assay, 384 Well)$4,449.00Add to Cart
3011-100KTranscreener ADP2 TR-FRET Red Assay (10 X 10,000 Assay, 384 Well)$22,245.00Add to Cart

Assay Plates for TR-FRET

Cat. No.Product DescriptionPriceAdd to Cart
2234Corning 96-Well White Assay Plates (#3642), 3-Pack (200+ Assays)$55.00Add to Cart
2231Corning 384-Well White Assay Plates (#4513), 3-Pack (1,000+ Assays)$65.00Add to Cart
2232Corning 384-Well White Assay Plates (#4513), 30-Pack (10,000+ Assays)$529.00Add to Cart

Related Resources

Related Posts
Transcreener ADP Assay Reviews

BellBrook Labs
5500 Nobel Drive, Suite 230
Madison, Wisconsin 53711 USA
(608) 443-2400

info@bellbrooklabs.com

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