The Transcreener® ADP2 Assay with TR-FRET readout uses displacement of a tracer by ADP to measure enzymatic activity. This disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
The assay produces an excellent signal at <10% ATP consumption for a broad range of initial ATP concentrations (0.1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs. The universal detection method allows screening of virtually any kinase in a high throughput format.
Used in over millions of well by pharma since 2006, Transcreener is the most extensively validated ADP detection method available backed by the best technical support in the industry.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.