Discover NSP13 Modulators with the Transcreener ADP2 NSP13 Assay
The Transcreener ADP² Assay directly measures the ADP produced by NSP13 (also known as nonstructural protein 13 or SARS-CoV-2 NSP13 Helicase). These ADP measurements allow researchers to effectively determine the activity of the NSP13 enzyme. The assay provides a powerful tool to screen compound libraries for NSP13 modulators to help find new therapeutic antivirals for SARS-CoV-2.
The kit comes complete with all the detection reagents required to measure activity. NSP13 enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme.
The Role of NSP13 As Therapeutic Target
The COVID-19 outbreak has led to intense research to find new therapeutics to help treat the disease. As a result, a variety of different targets are currently being explored to help fight the pandemic. As an antiviral target, NSP13 is critical for SARS-CoV-2 replication, thus making it an area of extreme interest to battle the disease.
As one of the 16 known coronavirus nonstructural proteins, NSP13 is the most similar to other forms of the virus, such as MERS-CoV and SARS-CoV, making it an exciting target not only for the current pandemic but for future outbreaks as well (White et al. 2020).
Direct Detection of ADP to Measure NSP13 Enzymatic Activity
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases