Discover OAS1 Modulators with Transcreener OAS1 Assay Services
The Transcreener OAS1 Assay measures the 2-5A produced by OAS1 (also known as 2'-5'-oligoadenylate synthetase 1). By measuring the production of OAS1 researchers can effectively determine the activity of the OAS1 enzyme. The assay provides a powerful tool to screen compound libraries for OAS1 modulators to help find new therapeutics for a variety of different conditions including autoimmune diseases.
The assay is currently available as a service performed in-house at BellBrook Labs. Please contact us for questions related to acquiring the assay.
The Role of OAS1 In Innate Immunity
Double stranded RNA enters the cell from viral infection. As a first line of defense in the innate immune system, OAS1 is activated by viral double-stranded RNA. Upon activation, OAS1 synthesizes 2-5A from ATP which in turn activates RNase L. The result of this signaling event cleaves both viral and host RNA and in turn inhibition of protein synthesis in the cell, thus ending the replication of invading RNA viruses. Mutation or inadvertent stimulation can produce autoimmune conditions by activating the pathway inconsistently even while not in the presence of viral invaders.
Detection of 2-5A to Measure OAS1 Enzymatic Activity
In the presence of dsRNA OAS1 uses ATP to produce the messenger 2-5A. The assay uses a coupling enzyme which generates AMP from the 2-5A. The AMP is then measured by the proven Transcreener AMP/GMP Assay with an FP readout.