Transcreener ADP2 PKR Assay
The Transcreener ADP² Assay directly measures the ADP produced by PKR (also known as Protein Kinase R, interferon-induced, double-stranded RNA-activated protein kinase, eukaryotic translation initiation factor 2-alpha kinase 2, or EIF2AK2). These ADP measurements allow researchers to effectively determine the activity of the PKR enzyme. The assay provides a powerful tool to screen compound libraries for PKR modulators to help find new therapies for disease.
The kit comes complete with the detection reagents required to measure activity. PKR enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme.
The Role of PKR As a Therapeutic Target
Protein Kinase R is a serine-threonine kinase with a central role in anti-viral activity through various PKR pathways. PKR decreases viral replication by reducing the phosphorylation of eukaryotic translation initiation factor (eIF2α), preventing further mRNA translation and protein synthesis. PKR also activates antiviral interferon cytokines through activation of nuclear factor kappa-light-chain-enhancers of activated B cells (NFkB) and induces apoptosis of viral cells, limiting spread of viral infection. The role PKR plays in anti-viral pathways has made it an emerging target for antiviral therapies. (Gal-Ben-Ari et al., 2019)
The Transcreener ADP² PKR Assay is an excellent tool for researchers examining the antiviral effects of PKR.
Direct Detection of ADP to Measure PKR Enzymatic Activity
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases