Discover TBK1 Inhibitors with the Transcreener ADP2 Assay
The Transcreener ADP² Kinase Assay directly measures the ADP produced by Tank Binding Kinase 1 (also known as TBK1). By measuring the production of ADP researchers can effectively determine the activity of the TBK1 enzyme. The assay provides a powerful tool to screen entire compound libraries for TBK1 modulators to help find new therapeutics for autoimmune diseases and cancer.
The Role of TBK1 As Therapeutic Target
More than 30 known proteins are known to interact with TBK1 on various levels. One of the most studied features is the innate immune response to foreign infectious material. Toll-like receptors activate the enzyme leading to further signaling. As a regulator of the immune response pathway to viral and bacterial material, TBK1 interacts with TANK and TRAF3. TBK1 then phosphorylates interferon regulator factors (IRFs), which activate inflammatory immune response with type I IFNs. Its role in inflammatory modulation makes TBK1 a critical target for autoimmune and neurodegenerative diseases. TBK1 also regulates aspects of cell proliferation and death, making it a key focus area in cancer research.
Direct Detection of ADP to Measure TBK1 Enzymatic Activity
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.