The Transcreener UDP² Glycosyltransferase Assay is a single step, homogenous, fluorescent assay for the detection and screening of UDP-producing enzymes. Direct detection of UDP with a fluorescence polarization (FP) or TR-FRET readout provides a safe, HTS-compatible alternative to cumbersome radioassay methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product. Based on BellBrook Lab’s proprietary nucleotide immunodetection technology, the Transcreener UDP² Glycosyltransferase Assay is compatible with any enzyme class that produces UDP, including UDP-glucose-, UDP-galactose- and UDP-glucuronosyl-transferases as well as glycogen, hyaluronan and cellulose synthases.

Learn how researchers at the Genesis Biotechnology group used the Transcreener UDP assay in a high throughput screen to discover  Clostridium difficile (C. difficile) toxin B (TcdB) inhibitors.