Homogenous Transcreener® UDP² TR-FRET Assay for High Throughput Screening with Glycosyltransferases
Direct Detection of UDP for Glycosyltransferases
About the Transcreener UDP2 Glycosyltransferase Assay Kits
The Transcreener UDP2 Glycosyltransferase Assay is a single step, homogenous, fluorescent assay for detection and screening of UDP-producing enzymes. Direct detection of UDP with a fluorescence polarization (FP) readout provides a safe, HTS-compatible alternative to cumbersome radioassay methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product. Based on BellBrook Lab’s proprietary nucleotide immunodetection technology, the Transcreener UDP2 Glycosyltransferase Assay is compatible with any enzyme class that produces UDP, including UDP-glucose-, UDP-galactose- and UDP-glucuronosyl-transferases as well as glycogen, hyaluranon and cellulose synthases.
Applications
- Screen compound libraries for inhibitors
- Hit confirmation
- IC50 Determination
- Determine inhibitor selectivity between enzymes
- Measure glycosyltransferase activity
Simple, “Mix-and-Read” Assay Format
Robust, HTS-Compatible Assay
Outstanding Deck and Signal Stability
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