A Homogenous Transcreener® UDP² TR-FRET Glycosyltransferase Assay for High Throughput Screening
About the Transcreener UDP2 Glycosyltransferase Assay Kits
The Transcreener UDP2 Glycosyltransferase Assay is a single step, homogenous, fluorescent assay for the detection and screening of UDP-producing enzymes. Direct detection of UDP with a fluorescence polarization (FP), TR-FRET, or all new FI readout provides a safe, HTS-compatible alternative to cumbersome radioassay methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product. Based on BellBrook Lab’s proprietary nucleotide immunodetection technology, the Transcreener UDP2 Glycosyltransferase Assay is compatible with any enzyme class that produces UDP, including UDP-glucose-, UDP-galactose- and UDP-glucuronosyl-transferases as well as glycogen, hyaluronan, and cellulose synthases.
Learn how researchers at the Genesis Biotechnology group used the Transcreener UDP assay in a high throughput screen to discover Clostridium difficile (C. difficile) toxin B (TcdB) inhibitors.
Direct Detection of UDP for Glycosyltransferases
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
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