The Transcreener® UDP2 Assay Kit with FI readout directly measures UDP via antibodies selective to UDP and a fluorescent tracer. Enzymatically generated UDP displaces the tracer from an antibody-quencher conjugate, resulting in increased fluorescence. The universal detection method allows screening of virtually any glycosyltransferase in a high throughput format.
The Transcreener UDP Assay Kit with FI readout produces a simple fluorescent intensity output which can be read on even the most basic fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers. Direct detection of UDP with a fluorescence intensity (FI) readout provides a safe, HTS-compatible alternative to cumbersome radioassay methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product.
Used in over millions of wells by pharma since 2006, Transcreener technology is the most extensively validated UDP detection method available backed by the best technical support in the industry.