Universal UDP-Glycosyltransferase Assay

The Transcreener UDP2 Assay is a single step, homogenous, fluorescent assay for detection and screening of UDP-producing enzymes. Direct detection of UDP with a fluorescence intensity (FI) readout provides a safe, HTS-compatible alternative to cumbersome radioassay methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product. Based on BellBrook’s proprietary nucleotide immunodetection technology, the Transcreener UDP2Assay is compatible with any enzyme class that produces UDP, including UDP-glucose-, UDP-galactose- and UDP-glucuronosyl-transferases as well as glycogen, hyaluronan and cellulose synthases.

Direct detection of UDP with no coupling enzymes means greater sensitivity with less interference.

Robust, HTS-Compatible Assay

  • Simple, “mix and read” assay format.
  • Far-red fluorescence minimizes interference.
  • Outstanding deck and signal stability.
  • Compatible with any multimode plate reader.

Excellent Z' With Low Levels of UDP Conversion

Validated on Most Commercially Available Plate Readers

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to ensure that Transcreener assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance.  We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

Supplier Instrument FI Assays
ADP2, GDP
bioteklogo TriStar²S LB 942 validated
Mithras² LB 943 validated
bioteklogo Cytation™ 5 validated
Cytation™ 3 validated
Cytation™ 1 validated
Synergy™ H1 validated
Synergy™ 2/H4/4 validated
Synergy™ HTX validated
Synergy™ Neo 2 validated
BMGLABTECH Logo POLARstar® Omega validated
FLUOstar® Omega validated
PHERAstar® FSX validated
PHERAstar® Plus/FS validated
CLARIOstar® /Plus validated
VANTAstar validated
MDS AT logo Analyst® GT/HT validated
Gemini® XPS/EM validated
SpectraMax® M2/M2e validated
SpectraMax® M5/M5e/FlexStation® 3 validated
SpectraMax® Paradigm contact us
perkinElmerLogo EnVision®/EnVision® Xcite validated
tecanLogo Infinite® M1000/M1000Pro/Safire2™ validated
Infinite® M200 validated
Infinite® F500 validated
Infinite® F200/Ultraevolution validated
Spark™ 10M validated

Validation Criteria

  • 384-Well Format
  • Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
  • Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
  • Z’ and Δ mP specifications to be met using Transcreener ADP2 Assay reagents
  • Read time to achieve Z’ and Δ mP specifications ≤ 5 minutes

Look for these stickers on instruments that have successfully met the validation criteria.

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