The Transcreener kinase assays that are available today have a range of benefits, and offer a great solution for many researchers around the world.. These and other Transcreener assays from BellBrook Labs have already been used to great effect by other organizations, and you can receive the same benefits from them. They are a competitive immuno assay, and provide you with a simple mix-and-read format.
How Can the Kinase Assay Be Used?
There are a range of applications where these kinase activity assays can be used. You can use them to screen for small molecule inhibitors, profile inhibitors and their potency, determine the enzymic activity, and more. Because it can provide direct detection of ADP, it will reduce the number of steps that might be found with other types of assays.
You will find that it is flexible, too. It is HTS ready, and it can be used in a range of well formats – 96, 384, or 1536. The concentrations used with the assay can vary, as well from 0.1 to 1000 µM.
Easy to Use
The mix-and-read format that is used with the kinase assay kits can be used in continuous or in endpoint modes. The continuous mode tends to be a good option for assay development, and it can be used when trying to find the residence times for kinase inhibitors. The endpoint assays are typically used for HTS. Automation is simplified, and the kinase activity assays have a substantial amount of flexibility.
It Provides Stability
This kinase assay option is also going to provide users quality reagents and signal stability. The antibody and tracers are capable of remaining stable at room temperature for a minimum of eight hours. This means that you do not have to worry about reading the plates immediately. It also means that if the reagents are at room temperature for a few hours before being used, it will not cause a problem, and the results are still going to be accurate.
Of course, one of the best reasons to use the Transcreener kinase assay from BellBrook Labs is because it is very accurate. They use a proprietary antibody that will bind with the ADP, which allows for fast and accurate ADP detection.