Learn More About Transcreener Method Used for This CD38 Assay

Discover CD38 Modulators with Transcreener CD38 Assay Services

The Transcreener CD38 Assay measures the ADPR produced by CD38 (also known as cluster of differentiation 38 or cyclic ADP ribose hydrolase). By measuring the production of CD38 researchers can effectively determine the activity of the enzyme. The assay provides a powerful tool to screen compound libraries for CD38 modulators to help find new therapeutics for a variety of different conditions including autoimmune diseases and cancer.

The assay is currently available as a service performed in-house at BellBrook Labs. Please contact us for questions related to acquiring the assay.

The Role of CD38 as a Therapeutic Target

CD38 is an ectoenzyme found on the surface of a variety of immune cells. It can also be located in other parts of the cell such as the mitochondria. Soluble CD38 is also present in the cytoplasm. As part of the extracellular CD38/CD203a/CD73/CD39 pathway which modulates adenosine production, CD38 is one of the first steps by producing ADPR from NAD+. Extracellular adenosine is a key player in the regulation of inflammation and immunogenicity. As such CD38 provides a potential target for tumor evasion and inflammatory-related disease. Intracellular CD38 is also a target for asthma as cADPR-mediates calcium channels that control smooth muscle associated with breathing.

Detection of ADPR to Measure CD38 Enzymatic Activity

Transcreener CD38 Assay Schematic

CD38 uses NAD+ to produce the ADPR and cADPR. The assay uses a coupling enzyme that generates AMP from the ADPR produced. The AMP is then measured by the proven Transcreener AMP Assay with an FP readout.


  • Measure Enzymatic Activity of CD38
  • Screen Compound Libraries for CD38 Modulators
  • Quantify Inhibitor Potency
  • Inhibitor Selectivity Profiling


  • Designed as in homogenous, one-step format to enable reproducibility.
  • Robust Assay Z’ > 0.7 under initial velocity conditions
  • Far-red fluorescent readouts minimize compound interference
  • Available in FP, FI, or TR-FRET readouts depending on your requirements

Mix-and-Read, HTS-Ready Assay, for Robust Screening

The Transcreener CD38 Assay is in an HTS-ready, mix-and-read format. We'll run your enzyme reaction, add Transcreener reagents, and read the plates. The assay is compatible with 96, 384, and 1536-well formats.

Transcreener Mix and Read Assay

Enzyme Titration with the Transcreener CD38 Assay - FP Readout

The assay demonstrates linearity when raw data is converted to the product formed using a standard curve. Linearity is shown under initial velocity conditions when raw data is converted to AMP formed as ADPR is broken down by the detection reagents.

Human CD38 Enzyme Titration

CD38 Enzyme Titration

Data Converted to Product Formed

CD38 Enzyme Titration Converted Data

Screen for Inhibitors & Perform SAR

Transcreener Assays are designed for screening compound libraries in a high throughput format. Follow-up SAR can also be performed using the assay to determine inhibitor potency both quickly and accurately.

Robust Assay Yields Quality Results

Z' CD38 Assay

Accurate Dose-Response Data

Dose-response curve with CD38 Assay

IC50 = 0.00803 μM

CD38 Assay Services

Interested in moving your program forward, but don't want to bring an assay in-house? Our scientists can help! BellBrook scientists will use their extensive biochemistry and enzymology expertise to work with you and accelerate your CD38 or innate immunity program.

Assay Development Services

Lead Discovery Services Include:

  • Inhibitor Screening – To identify or confirm activity with the target.
  • Inhibitor Potency Profiling – Dose-response with target and/or related proteins. Fast IC50 results.
  • Mechanism of Action Studies – Kinetic analysis to define the mode of inhibition.

Contact Us to Learn More

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