GTPase Profiling Services | BellBrook Labs

BellBrook's Scientists Use the Proven Transcreener GDP Assay to Directly Measure the Activity of GTPase Enzymes as Well As GAPs and GEFs to Get You Accurate Results Fast

Interested in moving your program forward, but don't want to bring an assay in-house? Our scientists can help! BellBrook scientists right here in Madison, Wisconsin will use their extensive biochemistry and enzymology expertise to work with you and accelerate your GTPase program. We can provide inhibitor potency profiling for a variety of GTPase enzymes and related proteins. Get accurate IC₅₀ results fast, to understand how your inhibitor interacts with other GTPases. Contact us today to see if BellBrooks's GTPase profiling services will advance your research.

Standard GTPase Profiling Menu

Choose from this menu or inquire about other GTPases, GAPs, and GEFs.

GTPase Family	Enzyme	Enzyme Type
Ras Family	KRas	Wild Type
	KRas (G-13D)	Mutant
	KRas (G-12C)	Mutant
	KRas (G-12V)	Mutant
	KRas (G-12D)	Mutant
	HRas	Wild Type
	NRas	Wild Type
	RRas	Wild Type
Rho Family	RhoA	Wild Type
	RhoC	Wild Type
	Cdc42	Wild Type
	Rac1	Wild Type
Ran Family	RanT24N	Mutant
Rab Family	Rab2	Wild Type

How It Works

GTPase Enzyme Reaction

Measure Activity with the Transcreener GDP Assay

GTPase Profiling Services Assay Schematic

Quickly Provide a Report & Support

Contact Us to Learn More About GTPase Profiling Services

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Related Resources

Poster Presentations

A Homogenous, High-Throughput Assay to Measure Guanine Nucleotide Exchange Factor Activity Using Transcreener GDP Assay (SLAS 2017)
Interrogation of Modulators of GPCR and small GTPase Activity: Screening GAPs and GEFs with the Transcreener GDP Assay (DOT 2015)
A High Throughput Assay for Rho Guanine Nucleotide Exchange Factors Based on the Transcreener GDP Assay (FASEB Small GTPases 2015)
Selective Detection of LRRK2 GTPase Activity with the Transcreener GDP Assay (SLAS 2013)
Investigation of different GTPase classes with the Transcreener GDP Assay (SLAS 2012)
Discovery of small molecule inhibitors for RGS proteins using the RGS screen Platform (SBS 2011)
Targeting RGS Proteins using Engineered Galpha Proteins and the Transcreener GDP Assay (SBS 2010)
Engineering G alpha Proteins for Detection of RGS Protein GAP Activity with the Transcreener GDP Assay (Screening Europe 2009)
GDP Detection Using the Transcreener HTS Assay Technology (SBS 2008)
An RGS Protein HTS Strategy using the Transcreener GDP Assay (SBS 2008)

Publications

Journal of Biological Chemistry. 2019 Nov 8;294(45):16865-83.
L-type prostaglandin D synthase regulates the trafficking of the PGD2 DP1 receptor by interacting with the GTPase Rab4.
Binda C, Génier S, Degrandmaison J, Picard S, Fréchette L, Jean S, Marsault E, Parent JL.

Methods in Molecular Biology, 2018. vol 1821. Humana Press, New York, NY
High-Throughput Assay for RhoGEFs Based on the Transcreener^® GDP Assay. In: Rivero F. (eds) Rho GTPases.
Lowery R.G., Kumar M.

PloS one. 2017 Sep 28;12(9):e0185639.
A highly-sensitive high throughput assay for dynamin’s basal GTPase activity.
Mohanakrishnan A, Tran TV, Kumar M, Chen H, Posner BA, Schmid SL.

Journal of Virology. 2015 Nov 1;89(21):10879-90.
Functional comparison of Mx1 from two different mouse species reveals the involvement of loop L4 in the antiviral activity against influenza A viruses.
Verhelst J, Spitaels J, Nürnberger C, De Vlieger D, Ysenbaert T, Staeheli P, Fiers W, Saelens X.

J Biomol Screen. 2015 Jul 20. pii: 1087057115596326. [Epub ahead of print]
A High-Throughput Assay for Rho Guanine Nucleotide Exchange Factors Based on the Transcreener GDP Assay
Reichman M, Schabdach A, Kumar M, Zielinski T, Donover PS, Laury-Kleintop LD, Lowery RG.

Curr Protoc Pharmacol. 2012 Mar;Chapter 2:Unit2.8.
Evaluating Modulators of “Regulator of G-protein Signaling” (RGS) Proteins.
Bosch DE, Zielinski T, Lowery RG, Siderovski DP.

J Biomol Screen. 2011 Aug;16(7):717-23. Epub 2011 May 18
High-throughput fluorescence polarization assay for the enzymatic activity of GTPase-activating protein of ADP-ribosylation factor (ARFGAP).
Sun W, Vanhooke JL, Sondek J, Zhang Q.

J Biomol Screen. 2009.
Two Gα_i1 Rate-Modifying Mutations Act in Concert to Allow Receptor-Independent, Steady-State Measurements of RGS Protein Activity
Zielinski T, Kimple AJ, Hutsell SQ, Koeff MD, Siderovski DP, Lowery RG.

Literature

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