BellBrook's Scientists Use the Proven Transcreener GDP Assay to Directly Measure the Activity of GTPase Enzymes as Well As GAPs and GEFs to Get You Accurate Results Fast

Interested in moving your program forward, but don't want to bring an assay in-house? Our scientists can help! BellBrook scientists right here in Madison, Wisconsin will use their extensive biochemistry and enzymology expertise to work with you and accelerate your GTPase program. We can provide inhibitor potency profiling for a variety of GTPase enzymes and related proteins. Get accurate IC50 results fast, to understand how your inhibitor interacts with other GTPases. Contact us today to see if BellBrooks's GTPase profiling services will advance your research.

Assay Development Services

Standard GTPase Profiling Menu

Choose from this menu or inquire about other GTPases, GAPs, and GEFs.

GTPase FamilyEnzymeEnzyme Type
Ras FamilyK-RasWild Type
K-Ras (G-13D)Mutant
K-Ras (G-12C)Mutant
K-Ras (G-12V)Mutant
K-Ras (G-12D)Mutant
H-RasWild Type
N-RasWild Type
Rho FamilyRho AWild Type
CDC42Wild Type
Rac1Wild Type
Arf FamilyArf-1Wild Type
Arf -6Wild Type
Ran FamilyRanT24NMutant
Rab FamilyRab2Wild Type

How It Works

GTPase Enzyme Reaction

GTPase Activity Assay On Off Cycle

Measure Activity with the Transcreener GDP Assay

Quickly Provide a Report & Support

Download Our App Note

Contact Us to Learn More About GTPase Profiling Services

Please fill out the form below. We will respond quickly to get the conversation moving and learn how we can help. We keep things discrete, confidential, and professional.

Related Resources

Journal of Biological Chemistry. 2019 Nov 8;294(45):16865-83.
L-type prostaglandin D synthase regulates the trafficking of the PGD2 DP1 receptor by interacting with the GTPase Rab4.
Binda C, Génier S, Degrandmaison J, Picard S, Fréchette L, Jean S, Marsault E, Parent JL.

Methods in Molecular Biology, 2018. vol 1821. Humana Press, New York, NY
High-Throughput Assay for RhoGEFs Based on the Transcreener® GDP Assay. In: Rivero F. (eds) Rho GTPases.
Lowery R.G., Kumar M.

PloS one. 2017 Sep 28;12(9):e0185639.
A highly-sensitive high throughput assay for dynamin’s basal GTPase activity.
Mohanakrishnan A, Tran TV, Kumar M, Chen H, Posner BA, Schmid SL.

Journal of Virology. 2015 Nov 1;89(21):10879-90.
Functional comparison of Mx1 from two different mouse species reveals the involvement of loop L4 in the antiviral activity against influenza A viruses.
Verhelst J, Spitaels J, Nürnberger C, De Vlieger D, Ysenbaert T, Staeheli P, Fiers W, Saelens X.

J Biomol Screen. 2015 Jul 20. pii: 1087057115596326. [Epub ahead of print]
A High-Throughput Assay for Rho Guanine Nucleotide Exchange Factors Based on the Transcreener GDP Assay
Reichman M, Schabdach A, Kumar M, Zielinski T, Donover PS, Laury-Kleintop LD, Lowery RG.

Curr Protoc Pharmacol. 2012 Mar;Chapter 2:Unit2.8.
Evaluating Modulators of “Regulator of G-protein Signaling” (RGS) Proteins.
Bosch DE, Zielinski T, Lowery RG, Siderovski DP.

J Biomol Screen. 2011 Aug;16(7):717-23. Epub 2011 May 18
High-throughput fluorescence polarization assay for the enzymatic activity of GTPase-activating protein of ADP-ribosylation factor (ARFGAP).
Sun W, Vanhooke JL, Sondek J, Zhang Q.

J Biomol Screen. 2009.
Two Gαi1 Rate-Modifying Mutations Act in Concert to Allow Receptor-Independent, Steady-State Measurements of RGS Protein Activity
Zielinski T, Kimple AJ, Hutsell SQ, Koeff MD, Siderovski DP, Lowery RG.

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