Accelerating Discovery of Kinase Inhibitors Using an ADP Assay

Researcher Using An ADP Assay
An ADP assay can be used to study the activity of enzymes that phosphorylate substrates leading to signaling events in the body. One of a variety of post-translational modifications, protein phosphorylation regulates a diverse group of cellular functions. In cancer, abnormal phosphorylation events often play a role in proliferation, survival, motility, metabolism, and angiogenesis.1 PI3K
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A Search for NLRP3 Inhibitors to Fight Autoimmune Disease

Targeting Innate Immunity With NLRP3 Inhibitors
Can NLRP3 inhibitors help treat autoimmune disease? – The human immune response is complicated and we continue to discover pathways involved that add to the complexity. Different than the adaptive immune response, the innate response acts quickly and is considered the first line of defense against pathogens and foreign DNA. In order to do its
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Researchers Validate Hsp70 Activity with the Transcreener ATPase Assay

Researchers Studying Hsp70 Activity
Newly expressed human Hsp70 activity now being studied –  Heat shock protein 70 (Hsp70) is known for its upregulation during times of stress in order to maintain homeostasis. Like other heat shock proteins, Hsp70 is a chaperone protein that assists other proteins in correctly folding to become fully active molecules.  It binds to damaged, misfolded,
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ABC Transporter ATPase Assay Helps Discover MsbA Inhibitors

ABC Transporter ATPase Assay Inhibitor
MsbA Inhibitors Help Fight Against Gram-Negative Bacteria As pathogens continue to wreak havoc on human health, new therapeutics are needed. HTS compatible assays such as the Transcreener ABC Transporter ATPase Assay help pave the way to establish new treatments. Gram-negative bacterial pathogens pose grave dangers in clinical and environmental settings alike. They include foodborne disease
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Using an ATPase Activity Assay for Drug Discovery

atpase activity assays for drug discovery
What are ATPases? Many people are familiar with the abbreviation ATP, which stands for the molecule adenosine triphosphate. ATP is the fascinating energy carrier of cellular processes and is involved in energy transfer in all living organisms. The energy released upon the chemical transformation from ATP to ADP is used in a myriad of biochemical
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Acetyl-CoA Carboxylase Inhibitors: Transcreener ADP Assay Powers Drug Detection Strategy

Acetyl-CoA Carboxylase Inhibitors
The impact of Acetyl-CoA carboxylases (ACCs) on eukaryotic metabolism and metabolic-related disease states is profound. ACCs catalyze the formation of malonyl-CoA by ATP-dependent carboxylation of acetyl-CoA. In humans, the two isoforms of ACC exhibit highly regulated, tissue-specific patterns of expression, with ACC1 being present in lipogenic tissues such as liver and adipose, and ACC2 being
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