Investigating RAB2 as a Vesicle Transporter & Autophagy Initiator
Tuesday, 04 April 2023
RAB2 is part of the RAB family of small GTPases that regulate intracellular trafficking of membrane bound vesicles. It is highly conserved in eukaryotes, from C. elegans to humans. These GTPases influence the creation, movement, attachment, fusion, and destiny of vesicles originating in the endoplasmic reticulum (ER)/Golgi systems. Like other members of its family, RAB2
- Published in Emerging Targets, Innate Immunity
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Small GTPase Modulation: GDP Assays to Accelerate Discovery
Wednesday, 24 March 2021
Cancer is complex, which makes determining the culprit as well as individual treatment extremely challenging. A variety of cancer types exist with a multitude of contributors. As the second leading cause of death in the US, cancer has been a target scientists have been battling for decades. When DNA is damaged or mutations occur, cells
- Published in HTS Assays, Products
FUT7, Inflammation, and Fucosyltransferase Assays
Wednesday, 01 April 2020
Inflammation is a necessary step involved in the immune response, allowing our bodies to fight and eliminate disease, allergies, and injuries. The inflammatory response recruits several mediators to aid in the destruction of whatever pathogen caused the response in the first place. Cells such as leucocytes, eosinophils, and neutrophils get recruited to the site in
- Published in HTS Assays, Products
Researchers from BellBrook Labs Pen Chapter in Springer’s Rho GTPases: Methods and Protocols
Wednesday, 12 September 2018
New Protocol from BellBrook Labs: High-Throughput Assay for Rho GEFs Based on the Transcreener GDP Assay In this new second edition of Springer Nature’s Rho GTPases: Methods and Protocols, researchers from BellBrook Labs describe an HTS compatible method for detecting GTPase exchange factor (GEF) activity. The protocol described in chapter twelve of the book uses
New Posters from DOT 2017
Friday, 29 September 2017
Poster Presentations A Homogenous, High Throughput Assay to Measure Guanine Nucleotide Exchange Factor Activity Using Transcreener® GDP Assay Guanine nucleotide exchange factors (GEFs) positively regulate Rho GTPases by accelerating GDP dissociation to allow formation of the active, GTP-bound complex. Development of inhibitors that specifically disrupt GEF action on a target Rho GTPase may be a
- Published in HTS Assays, News
New Publication: HTS Method for Measuring Drug Residence Time
Tuesday, 25 April 2017
View our latest publication, “A High-Throughput Method for Measuring Drug Residence Time Using the Transcreener® ADP2 Assay” in SLAS Discovery. Inhibitor residence times are increasingly being used to prioritize molecules early in lead discovery programs. Current methods for determining residence time are low-throughput or require the synthesis of labeled ligands. Our goal was to create
- Published in Company, HTS Assays, News
Detecting Rho-GEF Activity Using the Transcreener® GDP Assay
Friday, 24 March 2017
Rho GTPases act as molecular switches to regulate the cytoskeletal dynamics underlying cell motility. Rho GEFs (guanine nucleotide exchange factors) positively regulate Rho GTPases by accelerating GDP dissociation and allowing formation of the active GTPase-GTP complex. Active Rho GTPases typically stimulate cell growth and migration, sometimes triggering tumorigenesis or invasion. Selectively disrupting GEF action using
- Published in Company, HTS Assays, Products
BellBrook Labs Awarded NIH Grant to Validate New Target for Liver Fibrosis Using Transcreener GTPase Assay Platform
Wednesday, 09 November 2016
BellBrook Labs was awarded a Phase I Small Business Innovative Research (SBIR) grant of $353,000 from the National Institute on Alcohol Abuse and Alcoholism for pharmacological validation of GIV, a nonreceptor guanine nucleotide exchange factor (GEF), as a therapeutic target for liver fibrosis. BellBrook will use proprietary high throughput GTPase assay methods to identify small
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