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Application Notes

BellBrook Labs offers informational application notes for our various Transcreener products. These notes provide a protocol and experimental sample for the use of the respective Transcreener assay to measure enzyme activity, screen compound libraries, profile inhibitors, measure residence time, and more!

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Application Notes: Transcreener ADP2 Assay

Determination of Drug-Kinase Residence Time with the Transcreener ADP2 Assay

Kinase Inhibitor Residence Time App Note

This Application Note provides a protocol and experimental example for the use of the Transcreener® ADP2 FP Assay to determine the residence time of a drug (or drug candidate) during its interaction with a kinase.

Simplified Protocol to Streamline Inhibitor Profiling with the Transcreener ADP2 Assay

ADP Assay Inhibitor Profiling Application Note

This Application Note describes how to streamline the process of inhibitor profiling with the Transcreener ADP2 FP Assay by eliminating the need to experimentally optimize the ADP2 Antibody concentration and the need for a standard curve.

Determination of KM for ATP & Kemptide with Protein Kinase A using Transcreener ADP2 Assay

ADP Assay Determination of Km Note Preview

This Application Note provides a protocol and example on how to use the Transcreener® ADP2 FP Assay to experimentally determine the KM value of Kemptide and ATP with Protein Kinase A (PKA) Enzyme.

Running Kinase Assays in Kinetic (Continuous) Mode with the Transcreener ADP2 Assay

App Note Running Kinases in Kinetic Mode

This Application Note describes running Transcreener's ADP2 Assay in kinetic mode. This method reduces the number of experiments required to optimize conditions for a new enzyme. Running assays in real-time save on the cost of reagents and provides a check on assay linearity.

Detection of ADK Activity with the Transcreener ADP2 Kinase Assay

Adenosine Kinase Application Note

This Application Note describes methods for using the Transcreener ADP2 Kinase Assay to measure ADK enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of MAPK8 Activity with Transcreener ADP2 Kinase Assay

MAPK8 Application Preview

This Application Note describes methods for using the Transcreener ADP2 Kinase Assay to measure MAPK8 enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of RIPK1 Activity with Transcreener ADP2 Kinase Assay

App Note Detection of RIPK1

This Application Note describes methods for using the Transcreener ADP2 Kinase Assay to measure RIPK1 enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of RIPK2 Activity with Transcreener ADP2 Kinase Assay

RIPK2 Activity Assay App Note Preview

This Application Note describes methods for using the Transcreener ADP2 Kinase Assay to measure RIPK2 enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of PKR Activity with Transcreener ADP2 Kinase Assay

PKR Application Note Preview

This Application Note describes methods for using the Transcreener ADP2 Kinase Assay to measure PKR enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of MAPK1 Activity with Transcreener ADP2 Kinase Assay

MAPK1 Application Note Preview

This Application Note describes methods for using the Transcreener ADP2 Kinase Assay to measure MAPK1 enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Application Notes: Transcreener GDP Assay

Direct Detection of GAP Activity using the RGScreenTM Platform to Discover Small Molecule Inhibitors for RGS Proteins

This Application Note describes how the RGScreenTM platform uses proprietary G alpha protein variants with altered GTP hydrolysis & GDP dissociation rates that enable detection of RGS GAP activity using the Transcreener GDP Assay.

Application Notes: AptaFluor Methyltransferase Assay

Detection of METTL3/METTL14 Activity with the AptaFluor SAH Methyltransferase Assay

Preview METTL3-METTL14 Application Note

This application note describes methods for using the AptaFluor SAH Methyltransferase Assay to measure METTL3/METTL14 enzymatic activity in a high-throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of PRMT5 Activity with the AptaFluor SAH Methyltransferase Assay

PRMT5 Application Note Preview

This application note describes methods for using the AptaFluor SAH Methyltransferase Assay to measure PRMT5 enzymatic activity in a high-throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of MLL4 Activity with the AptaFluor SAH Methyltransferase Assay

MLL4 Application Note Preview

This application note describes methods for using the AptaFluor SAH Methyltransferase Assay to measure MLL4 enzymatic activity in a high-throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Detection of NSP14 Activity with the AptaFluor SAH Methyltransferase Assay

NSP14 Application Note Preview

This application note describes methods for using the AptaFluor SAH Methyltransferase Assay to measure NSP14 enzymatic activity in a high-throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Application Notes: Transcreener EPIGEN Methyltransferase Assay

Inhibitor Dose Response Measurement with the EPIGEN Methyltransferase Assay Kit

EPIGEN Dose Response Measurement Application

This Application Note describes how to generate a dose response curve for an inhibitor using a Transcreener EPIGEN Assay kit. The general protocol shows how to set up an experiment to run dose response curves using Sinefungin as an example for HMT SET7/9 to determine the IC50 value.

Z' Determination with the EPIGEN Methyltransferase Assay Kit

Z' Determination for Transcreener EPIGEN Assay

This Application Note describes how to determine Z', the statistical parameter used to measure HTS quality, using a Transcreener EPIGEN Methyltransferase Assay kit. The general protocol shows how to set up an experiment to determine Z' value, using HMT PRMT3 as an example.

Detection of HMT G9a with the Transcreener EPIGEN Methyltransferase Assay

App Note Detection of HMT G9a EPIGEN Assay

This Application Note will serve as a guide for using the Transcreener EPIGEN Methyltransferase Assay to detect the initial velocity enzyme activity of HMT G9a with an assay window suitable for inhibitor screening and dose response measurements.

Detection of HMT SET7/9 with the Transcreener EPIGEN Methyltransferase Assay

Detection of SET7 9 EPIGEN Assay

This Application Note will serve as a guide for using the Transcreener EPIGEN Methyltransferase Assay to detect the initial velocity enzyme activity of HMT SET7/9 with an assay window suitable for inhibitor screening and dose response measurements.

Detection of HMT SET8 with the Transcreener EPIGEN Methyltransferase Assay

Detection of SET8 EPIGEN Assay

This Application Note will serve as a guide for using the Transcreener EPIGEN Methyltransferase Assay to detect the initial velocity enzyme activity of HMT SET8 with an assay window suitable for inhibitor screening and dose response measurements.

Detection of HMT PRMT1 with the Transcreener EPIGEN Methyltransferase Assay

Detection of PRMT1 EPIGEN Assay

This Application Note will serve as a guide for using the Transcreener EPIGEN Methyltransferase Assay to detect the initial velocity enzyme activity of HMT PRMT1 with an assay window suitable for inhibitor screening and dose response measurements.

Detection of HMT PRMT3 with the Transcreener EPIGEN Methyltransferase Assay

Detection of PRMT3 EPIGEN Assay

This Application Note will serve as a guide for using the Transcreener EPIGEN Methyltransferase Assay to detect the initial velocity enzyme activity of HMT PRMT3 with an assay window suitable for inhibitor screening and dose-response measurements.

Application Notes: Transcreener dAMP Exonuclease Assay

Detection of WRN Exonuclease Activity with the Transcreener dAMP FP Assay

Preview of WRN Exonuclease Application Note

This application note describes methods for using the Transcreener dAMP FP Assay to measure WRN exonuclease enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Application Notes: Transcreener ADPR Assay

Detection of SARM1 Activity with Transcreener ADPR FP Assay

SARM1 Application Note Preview

This application note describes methods for using the Transcreener ADPR FP Assay to measure SARM1 enzymatic activity in a high throughput (384-well) format, e.g., for inhibitor screening and/or inhibitor dose-response measurements.

Additional Application Notes

Settings to Validate BMG LABTECH CLARIOstar Plate Readers with Transcreener TR-FRET Assays

Application Note CLARIOstar Reader

This Application Note provides the optimal instrument parameters used to validate the BMG Labtech CLARIOstar Microplate Readers with Transcreener's ADP2 TR-FRET Assay & Transcreener's AMP2/GMP2 TR-FRET Assay.

Interpolating Data from a Standard Curve to Generate a Dose-Response Using GraphPad Prism

Preview of Data Analysis Application Note

This application note demonstrates how to analyze data utilizing GraphPad Prism software. It walks through the transformation, interpolation, and plotting of data generated during drug screening and inhibitor dose response profiling.

Application Notes: Transcreener AMP2/GMP2 Assay

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