The instrument validation program is a collaborative effort with the major suppliers of multimode readers to ensure that Transcreener assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance. We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.
| Supplier | Instrument | FP Assays ADP2, AMP2/GMP2, GDP, UDP, EPIGEN SAH, cGAMP |
FI Assays ADP2, GDP |
TR-FRET Assays ADP2, AMP2/GMP2, GDP, UDP, AptaFluor SAH, cGAMP |
|---|---|---|---|---|
 |
TriStar²S LB 942 | in review | validated | validated |
| Mithras² LB 943 | in review | validated | validated | |
 |
Cytation™ 5 | validated | validated | validated |
| Cytation™ 3 | validated | validated | validated | |
| Cytation™ 1 | validated | validated | validated | |
| Synergy™ H1 | validated | validated | validated | |
| Synergy™ 2/H4/4 | validated | validated | validated | |
| Synergy™ HTX | not capable | validated | not capable | |
| Synergy™ Neo 2 | validated | validated | validated | |
 |
POLARstar® Omega | validated | validated | validated |
| FLUOstar® Omega | not capable | validated | validated | |
| PHERAstar® FSX | validated | validated | validated | |
| PHERAstar® Plus/FS | validated | validated | validated | |
| CLARIOstar® /Plus | validated | validated | validated | |
 |
Analyst® GT/HT | validated | validated | validated |
| Gemini® XPS/EM | not capable | validated | not capable | |
| SpectraMax® M2/M2e | not capable | validated | not capable | |
| SpectraMax® M5/M5e/FlexStation® 3 | not capable | validated | validated | |
| SpectraMax® Paradigm | validated | contact us | contact us | |
 |
EnVision®/EnVision® Xcite | validated | validated | validated |
 |
Infinite® M1000/M1000Pro/Safire2™ | validated | validated | validated |
| Infinite® M200 | not capable | validated | not capable | |
| Infinite® F500 | validated | validated | validated | |
| Infinite® F200/Ultraevolution | contact us | validated | contact us | |
| Spark™ 10M | validated | validated | validated | |
Validation Criteria
- 384-Well Format
- Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
- Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
- Z’ and Δ mP specifications to be met using Transcreener ADP2 Assay reagents
- Read time to achieve Z’ and Δ mP specifications ≤ 5 minutes
Look for these stickers on instruments that have successfully met the validation criteria.
