- Transcreener® HTS Assays
- AptaFluor® HTS Assays
- HTS Assays by Target
- Ordering Information
The ADP FP Assay window at 10% conversion (0.5 µM ADP). The recommended enzyme concentration was an EC80 of 20 ng/mL. The assay demonstrated a Z' of 0.87 and a lower limit 0f detection at 0.06 μM ADP under these conditions.
ADP/ATP standard curves for a broad range of initial ATP concentrations (0.1 μM to 1000 μM ATP) using the FP, TR-FRET and FI assay respectively. ADP was increased as total ATP + ADP was held constant. Antibody was adjusted based on a linear relationship between EC85 and initial ATP. All assays were performed in 384-well plates (n=24) and read on the Tecan Safire 2 (FP), BMG Labtech PHERAstar Plus (TR-FRET), or the Perkin Elmer EnVision (FI) multimode plate readers.
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