Discover ATPase Inhibitors with the Transcreener ADP2 Assay
Transcreener ATPase Assay Kits provide a sensitive, HTS capable platform as demonstrated in millions of wells and multiple publications. As a universal technology eliminating the need for laborious internal assay development, Transcreener is an ATPase activity assay that directly measures ADP produced by enzymatic ATP hydrolysis.
Ultra-Sensitive Assay Measures ATPase Activity
In this peer-reviewed article, learn how researchers at Cancer Research UK used the sensitivity of Transcreener as an HSP90 and HSP72 ATPase activity assay when the malachite green technique wasn't sensitive enough for their drug discovery.
ATPase Assay Principle - Direct ADP Detection with a Fluorescent Readout
- Screen compound libraries for enzyme inhibitors
- Determine inhibitor selectivity between enzymes
- Profile hits for inhibitor potency
- Measure enzymatic activity
- Determine residence times for inhibitors
Fewer False Positives
Direct detection of ADP reduces the degree of interference seen with coupling enzyme-based assays, lowering the number of false positives. Far-red tracers eliminate fluorescent compound interference. Reduce your time triaging false hits and spend it finding druggable leads.
Non Radio-Active ATPase Assay Method
Transcreener provides an accurate, dependable, safe alternative to radio-labeled assays. No more disposal concerns.
The universal nature of the assay allows for the screening of virtually any ATPase enzyme. Universal assays also provide an effective technique for difficult targets. Get ahead of the curve with assays ready for emerging targets.
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases