Direct detection of AMP and GMP provides many advantages compared to complexity of coupled assays. Transcreener assays are the only HTS capable assays available with direct detection of unlabeled AMP or GMP.
Fewer False Positives
Direct detection reduces the degree of interference seen with coupling enzyme based assays, lowering the number of false positives.
Stable Reagents and Assay Signal
Assay reagents are stable at room temperature for a minimum of 8 hours. The fluorescent signal stability of Transcreener Ligase and Synthetase Assays lasts at least 24 hours. This provides the flexibility necessary to perform large automated screens.
Mix-and-Read
Fewer steps mean faster results, limited interference, less robot programming, and a lower probability for error.
Flexible Format
Use 96 well, 384 well, or 1536 well plates depending on your desired format.
Robust and Repeatable
Z’ values of 0.7 or greater are common under initial velocity conditions.
