Transcreener® AMP²/GMP² Ligase and Synthetase Assay

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AMP2/GMP2 Assay for Screening Ligases, Synthetases and Phosphodiesterases

The Transcreener AMP2/GMP2 Assay kits detect the enzymatic product of a Ligase or Synthetase reaction. Save time trying to create a custom assay for your challenging target. The universal nature of the Transcreener platform allows HTS-ready assay for a variety of enzyme targets.

Example Targets Include:

Ligases: Ub and SUMO Ligases, DNA and RNA Ligases
Synthetases: Acyl CoA Synthetase, Amino-Acyl tRNA Synthetases, NAD Synthetase

Applications

  • Screen compound libraries for enzyme inhibitors
  • Determine inhibitor selectivity between enzymes
  • Profile hits for inhibitor potency
  • Measure enzymatic activity
  • Determine residence times for inhibitors
Phosphodiesterase Assay Kit Schematic AMP GMP Assay FP
Phosphodiesterase Assay Kit Schematic AMP GMP Assay TR-FRET

The Simplest HTS-Capable Assay

Direct detection of AMP and GMP provides many advantages compared to complexity of coupled assays. Transcreener assays are the only HTS capable assays available with direct detection of unlabeled AMP or GMP.

Fewer False Positives

Direct detection reduces the degree of interference seen with coupling enzyme based assays, lowering the number of false positives.

Stable Reagents and Assay Signal

Assay reagents are stable at room temperature for a minimum of 8 hours. The fluorescent signal stability of Transcreener Ligase and Synthetase Assays lasts at least 24 hours. This provides the flexibility necessary to perform large automated screens.

Mix-and-Read

Fewer steps mean faster results, limited interference, less robot programming, and a lower probability for error.

Flexible Format

Use 96 well, 384 well, or 1536 well plates depending on your desired format.

Robust and Repeatable

Z’ values of 0.7 or greater are common under initial velocity conditions.

Download Case Study

This Case Study helps you learn how to detect AMP formed by E1 alone or the complete SUMO ligase cascade in a homogenous, mix and read assay and determine the potency of SUMO ligase inhibitors.

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    Continuous Kinetic Mode Compatible

    Transcreener Assays can be used in kinetic mode, allowing the assay to be used in applications not possible with endpoint only assay methods.

    Measure Residence Times

    Measure the residence times for inhibitors with a simple biochemical assay to characterize leads with the greatest potential to become drugs.

    Streamlined Assay Development

    Using the assay in kinetic mode reduces the number of experiments required to optimize conditions for a new enzyme.

    Far Red FP & TR-FRET Readouts Validated on Major Multimode Readers

    Supplier Instrument FP Assays
    		 TR-FRET Assays
    bioteklogo TriStar²S LB 942 in review validated
    Mithras² LB 943 in review validated
    bioteklogo Cytation™ 5 validated validated
    Cytation™ 3 validated validated
    Cytation™ 1 validated validated
    Synergy™ H1 validated validated
    Synergy™ 2/H4/4 validated validated
    Synergy™ HTX not capable not capable
    Synergy™ Neo 2 validated validated
    BMGLABTECH Logo POLARstar® Omega validated validated
    FLUOstar® Omega not capable validated
    PHERAstar® FSX validated validated
    PHERAstar® Plus/FS validated validated
    CLARIOstar® /Plus validated validated
    MDS AT logo Analyst® GT/HT validated validated
    Gemini® XPS/EM not capable not capable
    SpectraMax® M2/M2e not capable not capable
    SpectraMax® M5/M5e/FlexStation® 3 not capable validated
    SpectraMax® Paradigm validated contact us
    SpectraMax® iD3/iD5 in review validated
    perkinElmerLogo EnVision®/EnVision® Xcite validated validated
    tecanLogo Infinite® M1000/M1000Pro/Safire2™ validated validated
    Infinite® M200 not capable not capable
    Infinite® F500 validated validated
    Infinite® F200/Ultraevolution contact us contact us
    Spark™ 10M validated validated

    Transcreener AMP²/GMP² Assay Kits

    * For custom or bulk orders (over 100,000 wells) please contact us (info@bellbrooklabs.com) for a quote.

    Assay Plates for FP

    Cat. No.Product DescriptionPrice 
    2233Corning 96-Well Black Assay Plates (#3686), 3-Pack (200+ Assays)$40.00Add to Cart
    2229Corning 384-Well Black Assay Plates (#4514), 3-Pack (1,000+ Assays)$45.00Add to Cart
    2230Corning 384-Well Black Assay Plates (#4514), 30-Pack (10,000+ Assays)$400.00Add to Cart

    Assay Plates for TR-FRET

    Cat. No.Product DescriptionPrice 
    2234Corning 96-Well White Assay Plates (#3642), 3-Pack (200+ Assays)$40.00Add to Cart
    2231Corning 384-Well White Assay Plates (#4513), 3-Pack (1,000+ Assays)$50.00Add to Cart
    2232Corning 384-Well White Assay Plates (#4513), 30-Pack (10,000+ Assays)$450.00Add to Cart

    BellBrook Labs
    5500 Nobel Drive, Suite 230
    Madison, Wisconsin 53711 USA
    (608) 443-2400

    info@bellbrooklabs.com

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