The Transcreener Ligase & Synthetase Assay determines ligase or synthetase enzyme activity by directly measuring the AMP/GMP formed by the enzyme using the Transcreener AMP2/GMP2 assay. By detecting AMP/GMP output, the assay provides a universal method to assess the activity of any AMP/GMP-producing enzyme in real-time.
Transcreener Ligase & Synthetase Assay technology uses a simple but highly effective method that consists of an antibody selective to AMP/GMP and a far-red fluorescent tracer. AMP/GMP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout.
The ligase & synthetase assay is available with FP and TR-FRET. It is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagent, and measure. The simplicity of the system yields robust results that also makes it extremely amiable to HTS.