The Advantages Of The Transcreener Phosphodiesterase Assay

There are many phosphodiesterase assay kits currently on the market. Comparing the various assays allows pharmaceutical companies to determine for themselves the advantages of using a Transcreener biochemical assay.

At BellBrook Labs, located in Madison, WI, we focus on developing HTS assays which can be used to streamline and enhance the drug discovery process. For over 15 years we have developed quality, reliable, and streamlined biochemical assays for screening enzymes in key target families. Not only is this beneficial for high throughput screening solutions, but it also helps pharmaceutical companies to move more quickly through the drug development process, by allowing them to perform SAR, profiling, and kinetic studies all with the same assay.

The Benefits

Comparing our phosphodiesterase assay to others available provides a clear picture of the benefits of our screening and drug discovery tools. This includes the ability of the assay to detect any AMP/GMP producing enzyme with a range of precursor substrates. Similar to our kinase assay kit, this is an extremely versatile screening tool.

Our assay method, has been developed as the only with direct detection of unlabeled GMP or AMP, which can be done without the necessity of using coupling enzymes. The assay has fluorescence polarization (FP), fluorescence intensity (FI), and TR-FRET readouts which are easily read on a multimode plate reader. The assay kit can be used to screen for hundreds of targets and be used for a variety of different applications.

As an HTS assay, Transcreener can be used to determine the activity of the enzymes, measure inhibitor potency, and to also discover inhibitor selectivity for different targets within a target family. It is ideal to use as a tool to screen large compound libraries for inhibitors.

For more information on the PDE assay as well as our biochemical kinase assay products, contact us today.